A draw is no good for either side as both will be eliminated if they finish on two points, but Scotland will be a tough nut to crack.
Steve Clarke’s men will look to become the first national team in their history to reach the second round of a major tournament, so it will be some night in Glasgow.
Here’s everything you need to know ahead of the game.
When is the match?
The game kicks off at 8pm on Tuesday 22 June at Hampden Park.
Where can I watch it?
The match will be shown live on ITV2 and S4C, and can be streamed via the ITV Hub.
What is the team news?
Clarke is without Billy Gilmour and could opt for either Ryan Christie or David Turnbull to replace him.
Croatia are not said to have any injury issues heading into their final Group D encounter.
Croatia XI: Livakovic, Perisic, Lovren, Kovacic, Modric, Brozovic, Vlasic, Petkovic, Vida, Juranovic, Gvardiol
Scotland XI: Marshall; McTominay, Hanley, Tierney; O’Donnell, Armstrong, McGregor, Robertson; McGinn; Dykes, Adams
Croatia - 23/20
Draw - 11/4
Scotland - 2/1
Scotland can make history by reaching the second round of a major tournament for the first time. But while Croatia are not the team they were three years ago, they still have more pedigree than the Scots at the European Championships and also need a win to qualify. 1-1.